And so it begins…
This semester proves to be an interesting one in the research laboratory. Up to the moment I plan to pick up where I left off last semester with regards to reaction kinetics. Me and my mentor are planning to execute the sulfmyoglobin synthesis reaction under certain parameters, and this will definitely be taking a few saturdays and sundays out of my time. See, the problem is that last semester the kinetic study focused on the sulfmyoglobin synthesis reaction. The oxidation state of myoglobin was a mixture of oxy-mb and met-mb. My first assignment for this semester is to “re-do” the synthesis but I will be adding sodium dithionite to the stock Mb solution. This will help the Mb be in a uniform oxidation state (most importantly being oxy). By doing this I will be able to see if the formation constant is altered significantly.
Once I get that big question out of the way; then I will be studying the kinetics of the sulfmyoglobin synthesis reaction in the presence of hydrogen peroxide. This should yield some interesting results. As for equipment, it looks like I will be wrestling with the Apollo analyzer once more.
I should have more to share by the end of this month.
C-ya soon !

Mucho exito!